ChemBioChem · 2015 · 97 citations · 25 references
Life SciencesMicrobiologyMolecular BiologyGenetic EngineeringMicrobial BiotechnologyNatural Product BiosynthesisBiosynthetic Gene ClustersMicrobial Metabolite ProductionMicrobial Natural Product BiosynthesisPromoter EngineeringSynthetic Promoter DesignBacterial Natural Product BiosynthesisBiosynthetic Gene Cluster AnalysisConserved Gene ClustersMicrobial Genome-encoded BiosynthesisNatural Product Biosynthesis Gene DiscoveryNatural Product Gene Cluster IdentificationPromoter ControlPromoter Silencing
Exchange of the native promoter to the arabinose-inducible promoter PBAD was established in entomopathogenic bacteria to silence and/or activate gene clusters involved in natural product biosynthesis. This allowed the "on-demand" production of GameXPeptides, xenoamicins, and the blue pigment indigoidine. The gene clusters for the novel "mevalagmapeptides" and the highly toxic xenorhabdins were identified by this approach.
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