In vitro nematicidal activity of a terrestrial cyanobacterium, Synechococcus nidulans, towards plant-parasitic nematodes

Abstract

The nematicidal activity of a terrestrial cyanobacterium, Synechococcus nidulans , was investigated. Extracts of S. nidulans cultures collected at weekly intervals for 5 weeks were sonicated and tested against second-stage juveniles (J2) of Meloidogyne incognita. Extracts of 2-week-old cultures caused the maximum immobility (94.2%) and mortality (29.3%) of J2, compared with controls (medium and water). This extract was tested in vitro against infective stages and hatch of M. graminicola , Heterodera cajani , H. avenae and Rotylenchulus reniformis . Extracts of sonicated S. nidulans caused a mean immobility in the range of 91.3-98.4% in infective stages of the nematodes, with no significant difference with an increase in exposure time from 24 to 72 h. The greatest mean percentage mortality was observed in M. graminicola (31.5%) followed by M. incognita (29.3%), H. avenae (20.9%), and R. reniformis and H. cajani (both 17.4%) with a significant increase with the period of exposure from 24 to 72 h. No significant differences in mortality were observed between M. graminicola and M. incognita and between H. avenae and H. cajani. The percentage hatch inhibition over control (water) was greatest in M. incognita (94.2%), followed by H. avenae (91.6%), H. cajani (72.3%) and M. graminicola (70.6%), and least in R. reniformis (58.6%).