An enzyme‐linked immunosorbent assay (Elisa) for the detection of chlamydial antibody in duck sera

R.T. Evans, W.S.K. Chalmers, Peter R. Woolcock, H. Farmer, D Taylor‐Robinson

Avian Pathology · 1983 · 19 citations · 18 references

Concepts

Abstract

An enzyme-linked immunosorbent assay (ELISA) for the detection of chlamydial antibody in duck sera is described. The assay was used to test sera from a flock of domestic Pekin ducks infected with Chlamydia psittaci, and sera from wildfowl (Mallard). Sera from 63% of the domestic birds and 75% of the wildfowl exhibited chlamydial antibody titres of >/= 1/32, indicative of current or past chlamydial infection. There was a good correlation between the results obtained with the ELISA and those obtained by a micro-immunofluorescence test. The application of the ELISA to large scale screening of flocks is suggested.

References

18