CULTIVATION OF THE VIRUS OF LYMPHOGRANULOMA INGUINALE AND ITS USE IN THERAPEUTIC INOCULATION

Abstract

Heretofore, as far as I can learn,¹attempts to cultivate the etiologic agent of lymphogranuloma inguinale have been failures. I have been successful by utilizing the medium devised by Maitland, Laing and Lyth²for the cultivation of vaccinia virus. This medium is made by placing sterile bits of rabbit tissue in Tyrode's solution. I have used guinea-pig tissue instead of rabbit. <h3>METHOD OF CULTIVATION</h3> When pus is removed aseptically from a bubo that has not been exposed to external contamination and is found to be bacteriologically sterile, it is diluted 1: 5 with sterile saline solution. Now, when from 0.02 to 0.03 cc. of the diluted pus is planted in the Tyrode's solution containing a piece of guinea-pig kidney or liver and incubated at 37.5 C., a peculiar cloudiness appears throughout the supernatant fluid in from thirty-six to forty-eight hours. Control tubes of the medium incubated at the