MICROCALORIMETRIC STUDIES ON THE FORMATION OF MAGNESIUM COMPLEXES OF ADENINE NUCLEOTIDES

Abstract

Values for the thermodynamic quantities (DeltaF, DeltaH, DeltaS) in reactions in which complexes of adenine nucleotides with magnesium ion (ATPMg(--), ADPMg(-), AMPMg) are formed have been obtained by a microcalorimetric technique by using an isothermic Calvet's apparatus. Experimental values measured at ionic strength mu = 0.2 indicate that complex formation reactions are driven by the entropic factor and that stability of complexes increases with length of the phosphate chain.