Science · 1999 · 142 citations · 42 references
Life SciencesBiochemistryMolecular BiologyStructural BiologyTranscriptionTranscriptional RegulationMacromolecular AssemblyProtein Complex AssemblyRna Polymerase Ii TranscriptionRna Polymerase StructureTranscription In EukaryotesTranscription InitiationEukaryotic Rna Polymerase Ii StructureInitiation Complex StructurePromotersRna Polymerase Ii InitiationStructural Studies Of Initiation ComplexesTranscription Factor IiaTranscription Factor InteractionsTranscription Initiation Complex
The multisubunit transcription factor IID (TFIID) is an essential component of the eukaryotic RNA polymerase II machinery that works in concert with TFIIA (IIA) and TFIIB (IIB) to assemble initiation complexes at core eukaryotic promoters. Here the structures of human TFIID and the TFIID-IIA-IIB complex that were obtained by electron microscopy and image analysis to 35 angstrom resolution are presented. TFIID is a trilobed, horseshoe-shaped structure, with TFIIA and TFIIB bound on opposite lobes and flanking a central cavity. Antibody studies locate the TATA-binding protein (TBP) between TFIIA and TFIIB at the top of the cavity that most likely encompasses the TATA DNA binding region of the supramolecular complex.
42
Five intermediate complexes in transcription initiation by RNA polymerase II
Stephen Buratowski, Steven Hahn, Leonard Guarente et al. · Cell · 1989 · 1K citations