Preliminary crystallographic analysis of chloroperoxidase from<i>Caldariomyces fumago</i>

Abstract

Chloroperoxidase from the fungus Caldariomyces fumago has been crystallized in two space groups, C222(1) and P2(1)2(1)2(1) both of which are suitable for high-resolution X-ray studies. Parent data sets have been obtained to 2.16 A in space group C222(1) and 2.00 A in space group P2(1)2(1)2(1). Heavy-atom derivatives have been obtained with both forms and electron-density maps calculated. The heme has been located and continuous electron density between the heme and protein clearly indicates the location of the proximal ligand.