Transactions of the Royal Society of Tropical Medicine and Hygiene · 1984 · 62 citations · 14 references
Life SciencesInfectious DiseasesMicrobiologyMedical MicrobiologyMicrobial EcologyParasitologyPathogen DetectionPathogen IsolationCulture Media SelectionEntamoeba-host InteractionsGeographical DisparitiesOxidoreductase EnzymesSolid Culture Media
Stocks of Entamoeba histolytica isolated and maintained in a variety of culture media, both axenic and polyxenic, were compared with each other and with strains previously characterized. The present stocks were isolated from subjects living in various cities in India. Using the enzyme patterns of: E C 5319 glucose phosphate isomerase (GPI); E C 11140 L-malate: NADP+ oxidoreductase (oxaloacetate decarboxylating) (ME): E C 2751 phosphoglucomutase (PGM); and E C 2711 hexakinase (HK), developed after electrophoresis, three zymodemes were identified in 54 individual isolations of E. histolytica. Most importantly only one zymodeme associated with pathogenicity occurred, identified 28 times, among the collection, one of which was from a liver abscess.
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