In-line color digital holographic microscope for water quality measurements

Abstract

We introduce a color digital holographic microscope for measuring the biological content of water samples. Our approach uses single shot RGB exposure in an in-line holographic setup to obtain color images. With the application of appropriate numerical algorithms we can fulfill color crosstalk compensation, segmentation, and twin image removal tasks, and we obtain good quality color image reconstructions with 1μm resolution from a 1mm³ volume. We briefly compare the conventional color CCD/CMOS and the Foveon X3 sensor for color digital holographic applications. The in-line holographic setup and reconstruction algorithms are presented with demonstrative simulations, experimentally captured and numerically reconstructed images.