Isolation and estimation of the aggregation pheromone from Eysarcoris lewisi (Distant) (Heteroptera: Pentatomidae)

Abstract

A male-produced crude aggregation pheromone from Eysarcoris lewisi (Distant) (Heteroptera: Pentatomidae) was collected from volatiles emitted by adult males. It was purified with high performance liquid chromatography using a silica gel column, followed by preparative gas chromatography. The attraction activity of each fraction was field tested using water pan traps. The isolated compound of the aggregation pheromone component was analyzed by GC-MS and NMR, and the chemical structure was estimated to be (Z)-2-methyl-6-(4-methylenebicyclo[3.1.0]hex-1-yl)hept-2-en-1-ol.