Synthesis of phosphorothloate-contaming DNA fragments by a modified bydroxybenzotriazole phospbotriester approach

Abstract

The phosphorothioylating agent which was obtained by treating 2,5-dichlorophenyl phosphorodichloridothioate with 1-hydroxy-6-nitrobenzotriazole proved to be very effective for the synthesis in solution and on a solid support of phosphorothioate-containing DNA fragments.