Growth-Promoting Properties of Pyruvate, Oxalacetate, and -Ketoglutarate For Isolated Walker Carcinosarcoma 256 Cells

Abstract

When Walker carcinosarcoma 256 cells were grown under clonal growth technics. 50-100% plating efficiency was noted in a medium containing whole bovine serum. When dialyzed serum was used in the medium, few or no colonies were formed. Addition of pyruvate, α-ketoglutarate, and/or oxalacetate to the substrate resulted in plating efficiencies of 90-100%. Activity of these 3 metabolites was the same irrespective of addition of individual compounds or combinations thereof. Other Kreb's acids and metabolites tested failed to promote survival and growth of isolated Walker cells.