DETECTION OF HUMAN ANTIBODIES TO TRICHINELLA SPIRALIS BY ENZYME‐LINKED IMMUNOSORBENT ASSAY, ELISA

Abstract

Enzyme‐linked immunosorbent assay, ELISA, was used to detect and quantitate antibodies to Trichinella spiralis in human sera. An extract of Trichinella spiralis larvae was adsorbed to polystyrene tubes. These were incubated with serum and bound antibodies were detected by anti‐immunoglobulin labelled with alkaline phosphatase. Class specific conjugates were used to detect specific IgM and IgA antibodies. ELISA was at least as sensitive as passive haem‐agglutination and more sensitive than indirect immunofluorescence.