Rapid Communications in Mass Spectrometry · 1995 · 18 citations · 3 references
PharmacyChemistryPharmaceutical AnalysisAnalytical ChemistryChromatographic AnalysisMass Spectrometric AnalysisAnalytical ChromatographyChromatographyInstrumental AnalysisLiquid ChromatographyMass SpectrometryStructural Elucidation Of ImpuritiesAccuracyElectrospray IonizationFourier Transform Ion Cyclotron Resonance Mass SpectrometryImpuritiesLiquid Chromatography-mass SpectrometryLiquid Chromatography-mass Spectrometry AnalysisMass Spectrometric Drug AnalysisMass Spectrometric Structural Elucidation
Abstract The separation of trace impurities in cimetidine drug substance by high pressure liquid chromatography (HPLC) showed a series of minor components. These were analysed using an on‐line electrospray source connected to a Fourier transform ion cyclotron resonance (FT‐ICR) mass spectrometer. Working typically with a resolution of 10 000–20 000 accurate mass measurement of the components confirmed their empirical formulae to generally better than 5 ppm accuracy. This work shows that the combination of conventional analytical HPLC using 4.6 mm columns with a low flow rate electrospray source interfaced to an FT‐ICR mass spectrometer enables structural information to be obtained on low levels of low mass components.
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