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Expression of theRenilla reniformis Luciferase Gene in Mammalian Cells
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1996
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Transcriptional RegulationSignal TransductionProtein ExpressionRenilla Luciferase GeneMedicineNatural SciencesRenilla Reniformis LuciferaseReporter Gene AssayGene StructureMolecular BiologyMolecular Biological MethodMolecular GeneticsGene ExpressionMammalian CellsCell BiologyCellular PhysiologyGene FunctionGene Transfer
A cDNA encoding the Renilla reniformis luciferase was expressed in similan and murine cells in a transient and stable manner, respectively. Light emission catalyzed by luciferase was detected from transfected cells both in vitro and in vivo. This work establishes the Renilla luciferase gene as a new efficient marker of gene expression in mammalian cells.