Proceedings of the National Academy of Sciences · 1991 · 158 citations · 22 references
Life SciencesNeurosciencePharmacologyMolecular NeuroscienceNeurochemistryNeuropharmacologyCholinergic NeurochemistryCholinergic PharmacologyCholinergic SignalingGabaergic PharmacologyNeuropeptide PharmacologyNeurotransmitter ReceptorsAcetylcholine PharmacologyAntagonist ActivityEvoked Neurotransmitter ReleaseEvoked ReleaseGalanin PharmacologyMembrane HyperpolarizationNeuropeptide Binding AffinityPeptide Binding Affinity
The 20-amino acid peptide M-15 binds with high affinity (IC50 approximately 0.1 nM) to 125I-labeled galanin (125I-GAL) binding sites in membranes from the ventral hippocampus, midbrain, and rat spinal cord. Receptor autoradiographic studies show that M-15 can displace 125I-GAL from all labeled sites. M-15 acts as a reversible high-affinity antagonist in blocking the inhibitory effects of GAL on the evoked release of acetylcholine in vivo in the hippocampus and on the GAL-induced hyperpolarization of locus coeruleus neurons in slices. M-15 also blocks the facilitatory effects of GAL on the spinal flexor reflex. Thus, the chimeric peptide M-15 [GAL-(1-13)-substance P-(5-11)amide] represents the first antagonist to the neuronal actions of GAL.
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The rat brain in stereotaxic coordinates
Miklós Palkovits · Neuropeptides · 1983 · 29K citations
Medicine, Neurobiology, Neuroscience +12
Galanin — a novel biologically active peptide from porcine intestine
Kazuhiko Tatemoto, Åke Rökaeus, Hans Jörnvall et al. · FEBS Letters · 1983 · 1.5K citations · Full text