Journal of the American Chemical Society · 2004 · 443 citations · 17 references
Life SciencesBiochemistryBiotechnologyMagnetic MaterialsNanobiotechnologyProtein BiochemistryImmunomagnetic SeparationMagnetic NanomaterialsMagnetic SeparationNanoparticle DesignPeptide ChemistryProtein-based NanomaterialsMagnetic NanoparticlesMagnetic Support ImmobilizationNanoparticle-assisted SeparationNanoparticle-based ImmobilizationNanoparticlesPeptide NanoparticlesProtein Separation TechniquesProtein-nanoparticle Conjugation
Using Nalpha,Nalpha-bis(carboxymethyl)lysine to react with FePt magnetic nanoparticles, we synthesized the FePt-NTA conjugate, which immobilizes Ni2+ ions and selectively binds to histidine-tagged proteins at concentration as low as 0.5 pM. This simple system serves as a useful alternative to existing protocols for protein separation and also acts as a versatile agent for transporting and anchoring proteins.
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Metal chelate affinity chromatography, a new approach to protein fractionation
Jerker Porath, Jan Carlsson, Ingmar Olsson et al. · Nature · 1975 · 2.1K citations
Efficient Activation of Aromatic C-H Bonds for Addition to C-C Multiple Bonds
Chengguo Jia, Dongguo Piao, Juzo Oyamada et al. · Science · 2000 · 819 citations