A vernalization protocol for obtaining progenies from regenerated and transgenic tall fescue plants

Abstract

Abstract Tall fescue is an important outcrossing forage and turf grass species that requires vernalization to flower. A reproducible protocol was developed for vernalization of regenerated, transgenic and seed‐derived tall fescue plants. Following the vernalization scheme that involved gradual changes of temperature and daylength, seeds were routinely produced from vernalized plants under greenhouse conditions. Molecular analyses of progenies obtained from crosses between transgenic and seed‐derived plants revealed stable meiotic transmission of transgenes following Mendelian inheritance in transgenic tall fescue.