Nucleic Acids Research · 1986 · 178 citations · 17 references
Life SciencesMicrobiologyMolecular BiologyEnzymologyGene ExpressionGenetic EngineeringAntibiotic ResistanceAntibiotic Resistance GeneticsAntibiotic Resistance MechanismsEnzyme ExpressionGenetic Engineering Of MicroorganismsPlasmid GeneticsAntibiotic Resistance Gene TransferAntibiotic Resistance GenesBacterial Expression VectorsPlasmid Gene TransferResistance Plasmid TransferTransfection In Mammalian Cells
The gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger has been cloned next to the SV40 early promoter in a mammalian cells-Escherichia coli shuttle vector. When this construction was introduced into VERO cells it expressed the relevant enzymic activity. Moreover, the puromycin N-acetyl transferase gene has been used as a dominant marker for the selection of transformed mammalian cells able to grow in the presence of the antibiotic.
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