Concepedia

Evaluation of Diagnostic Tests for <i>Clostridium difficile</i> Infection

Jonathan Swindells, N. Brenwald, Nathan Reading, Beryl Oppenheim

Journal of Clinical Microbiology · 2009 · 152 citations · 10 references

DOIFull text

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Concepts

Abstract

We evaluated toxigenic Clostridium difficile detection by a lateral flow assay for antigen and toxin, an enzyme immunoassay, and two commercial PCR methods. Compared to the cell cytotoxicity neutralization assay and toxigenic culture, both toxin detection methods lacked sensitivity. PCR following combined antigen and toxin detection provided the most useful diagnostic information.

References

10

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Effective Detection of Toxigenic <i>Clostridium difficile</i> by a Two-Step Algorithm Including Tests for Antigen and Cytotoxin

John R. Ticehurst, Deborah Aird, Lisa Dam et al. · Journal of Clinical Microbiology · 2006 · 209 citations · Full text

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Comparison of a Commercial Real-Time PCR Assay for <i>tcdB</i> Detection to a Cell Culture Cytotoxicity Assay and Toxigenic Culture for Direct Detection of Toxin-Producing <i>Clostridium difficile</i> in Clinical Samples

Paul D. Stamper, Romina Alcabasa, Deborah Aird et al. · Journal of Clinical Microbiology · 2008 · 181 citations · Full text

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Yield of Stool Culture with Isolate Toxin Testing versus a Two-Step Algorithm Including Stool Toxin Testing for Detection of Toxigenic <i>Clostridium difficile</i>

Megan E. Reller, Clara Lema, Trish M. Perl et al. · Journal of Clinical Microbiology · 2007 · 155 citations · Full text

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Rapid and Reliable Diagnostic Algorithm for Detection of <i>Clostridium difficile</i>

Lukas Fenner, Andreas F. Widmer, Gisela Goy et al. · Journal of Clinical Microbiology · 2007 · 142 citations · Full text

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