FEBS Letters · 1987 · 260 citations · 8 references
Life SciencesBiochemistryMolecular BiologyNucleic Acid BiochemistryNucleic Acid ChemistryRna ProcessingNucleic Acid CleavageOligonucleotide ChemistryRibonucleic Acid (Rna) ChemistryRna ChemistryRna CleavageRna Modification2'-O-methyl Modification2'-O-methyl Oligonucleotides2'-O-methyl RnaHydrolysisOligonucleotide Cleavage ChemistryRibonuclease ChemistryRibonucleotide ChemistrySequence-specific Rna Cleavage
To cleave RNA molecules using RNase H in a site-specific manner, a short deoxyoligonucleotide (3-5mer) joining with 2'-O-methyl oligonucleotide(s) was designed as a DNA splint to be used. Model experiments were carried out using ribooligonucleotide substrates (9 and 18 mer). It was found that the use of this type of splints (9 mer) causes a unique cleavage by RNase H. For example, when 3'm (GA)d(AGAA)m(GGU)5' was used as a hybridization strand, 32pUCUUUCUUCUUCCAGGAU was cleaved specifically between U11 and C12 to yield 32pUCUUUCUUCUU. This method will have a variety of applications for the study of RNA.
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Excision of an intact intron as a novel lariat structure during pre-mRNA splicing in vitro
Barbara Ruskin, Adrian R. Krainer, Tom Maniatis et al. · Cell · 1984 · 765 citations
Site specific enzymatic cleavage of RNA
Helen Donis-Keller · Nucleic Acids Research · 1979 · 404 citations · Full text