Detection of HER2 breast cancer biomarker using the optofluidic ring resonator biosensor

Abstract

In this work, we describe a novel approach for detecting the HER2/neu extra-cellular domain (ECD) protein in human serum samples using the opto-fluidic ring resonator (OFRR). OFRR sensing technology that incorporates microfluidics and optical sensing methods to achieve rapid label free detection in a small and low cost platform. In this study, HER2 proteins were spiked in PBS running buffer and serum at varying concentrations. Concentrations of the HER2 protein were adjusted in serum to levels typical of breast cancer patients that show over-expression of this particular beast cancer biomarker. The OFRR was modified with a biologically functional layer to efficiently capture the HER2 biomarker and produce a sensing signal through interaction with the evanescent field of the optical resonator. Results show effective capture of HER2 at medically relevant concentrations in serum and was achieved for concentrations as low as 13 ng/mL and ranged to above 100 ng/mL. This work will lead to a device that can be used as a tool for monitoring disease progression in a low cost sensing setup.