The effect of inorganic phosphate on cyclic adenosine 3′, 5′-monophosphate in streptomycin-producing cultures of <i>Streptomyces griseus</i>

Abstract

Intracellular and extracellular cyclic adenosine 3′, 5′-monophosphate (cyclic AMP) levels were measured during the growth of a streptomycin-producing strain of Streptomyces griseus. Increasing the inorganic phosphate concentration of the defined media resulted in a decreased streptomycin titre and alterations in the magnitude and timing of changes in cyclic AMP concentration. Addition of excess inorganic phosphate after 24 h of growth reduced steptomycin production but had no effect on the intracellular cyclic AMP concentration. The results do not suggest that the effect of inorganic phosphate on streptomycin biosynthesis is mediated by cyclic AMP.