Biotin‐Functionalized Cellulose‐Based Monolayers as Sensitive Interfaces for the Detection of Single Molecules

Abstract

There's plenty of room on the microplate. We can immobilize and detect molecular recognition events over a broad concentration range by using laser-induced confocal single-molecule spectroscopy. For this purpose, we coated a glass-bottom microplate with a newly synthesized biotin–cellulose composite by dipping (Langmuir–Blodgett technique). Subsequent immobilization with streptavidin created a sensitive surface for capturing biotinylated targets.