Effects of the Change in Cutoff Values for Human Epidermal Growth Factor Receptor 2 Status by Immunohistochemistry and Fluorescence In Situ Hybridization: A Study Comparing Conventional Brightfield Microscopy, Image Analysis-Assisted Microscopy, and Interobserver Variation

Abstract

The change in cutoff levels led to a higher concordance between IHC and FISH, but fewer samples were classified as HER2 positive.