Genes to Cells · 1997 · 33 citations · 37 references
Life SciencesBiologyMolecular BiologyDna ReplicationGene ExpressionTranscriptional RegulationCell Cycle Gene RegulationG1 PhasePromoter RegulationReplication In EukaryotesS PhaseTranscriptional Regulation In EukaryotesCell Cycle DynamicsDna-binding MotifsG1 Phase ControlG1-s Phase TransitionProtein Subcellular LocalizationSubcellular Protein LocalizationYeast Gene Expression RegulationYeast Gene Regulation
BACKGROUND: The tight regulatory mechanism that prevents more than one round of chromosomal DNA replication per cell cycle is thought to require the function of Mcm/P1 proteins. We report here the structural and functional analyses of HsMcm6, a human homologue of the Mis5 of Schizosaccharomyces pombe. RESULTS: We demonstrate here that the transcription of the HsMCM6 gene was repressed in quiescent cells but was rapidly induced at the G1/S phase by growth factor stimulation. The 5' regulatory region of the HsMCM6 gene was found to harbour four putative E2F binding motifs, and these were responsible for the promoter activity. The HsMcm6 protein level oscillated during the cell cycle, with a peak at the G1/S phase. We also showed that the cell-cycle dependent change of subcellular localization of HsMcm6 resembles those of other Mcm/P1 proteins. HsMcm6 consists of two forms, a form extractable by Nonidet P-40 and the nucleus-bound form. A demonstration of the association of HsMcm6 with HsMcm2 and HsMcm7 in vivo supports the idea that they behave as a heteromeric complex. We mapped the HsMCM6 gene at 2q12-14. CONCLUSION: The results indicate that the behaviour of HsMcm6 is reminiscent of replication licensing factor like other Mcm/P1 family members.
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Antibodies, a laboratory manual
Biomedicine & Pharmacotherapy · 1989 · 12.5K citations
An improved PCR method for walking in uncloned genomic DNA
Paul D. Siebert, Alex Chenchik, D.E. Kellogg et al. · Nucleic Acids Research · 1995 · 1K citations · Full text