Manipulation of carbon assimilation with respect to expression of the pac gene for improving production of penicillin acylase in Escherichia coli

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Harry Towbin, T. Staehelin, J. Gordon · Proceedings of the National Academy of Sciences · 1979 · 53.8K citations · Full text

Life Sciences, Biochemistry, Molecular Biology +13

Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. 1977.

Francisco Bolívar, Rodriguez Rl, Greene Pj et al. · PubMed · 1992 · 4.7K citations

Life Sciences, Biotechnology, Genetic Engineering +8

Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid

Annie Chang, Stanley N. Cohen · Journal of Bacteriology · 1978 · 4.3K citations · Full text

Life Sciences, Genetics, Molecular Biology +17

A complementation analysis of the restriction and modification of DNA in Escherichia coli

Herbert W. Boyer, Daisy Roulland-Dussoix · Journal of Molecular Biology · 1969 · 3.9K citations

Life Sciences, Genetics, Microbiology +11

Construction and characterization of new cloning vehicle. II. A multipurpose cloning system

Francisco Bolívar, Raymond L. Rodriguez, Patricia J. Greene et al. · Gene · 1977 · 3.9K citations

Life Sciences, Biotechnology, Genetic Engineering +9