The effect of pore size of silica gel and concentration of buffer on capillary chromatographic and capillary electrochromatographic enantioseparations using cellulose tris(3,5-dichlorophenylcarbamate)

Abstract

A comparative study was performed on the effect of the pore size of silica gel and the concentration of the buffer on enantioseparations in capillary liquid chromatography (CLC) and capillary electrochromatography (CEC). Cellulose tris(3,5-Dichlorophenylcarbamate) was used as chiral selector in combination with nonaqueous methanolic buffers. Thiourea has proven not to be a suitable marker for the electroosmotic flow (EOF) in the present separation system. Optimization of the separation conditions led to plate numbers above 400 000 per meter as well as almost baseline enantioseparations with an analysis time of less than 1 min for the first time in chiral CEC with packed capillaries.