Concepedia

Construction of recombinant sake yeast containing a dominant FAS2 mutation without extraneous sequences by a two-step gene replacement protocol

Rinji Akada, Kazuyoshi Matsuo, Kazuo Aritomi, Yoshinori Nishizawa

Journal of Bioscience and Bioengineering · 1999 · 52 citations · 24 references

DOI

Concepts

References

24

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

R Sikorski, P. Hieter · Genetics · 1989 · 8.8K citations · Full text

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Transformation of intact yeast cells treated with alkali cations

Hisao Ito, Y Fukuda, Kousaku Murata et al. · Journal of Bacteriology · 1983 · 7.7K citations · Full text

, , +10

[19] Targeting, disruption, replacement, and allele rescue: Integrative DNA transformation in yeast

Rodney Rothstein · Methods in enzymology on CD-ROM/Methods in enzymology · 1991 · 1.3K citations

, , +9

Methods in Yeast Genetics — A Laboratory Course Manual

Alan Radford · Biochemical Education · 1991 · 1.3K citations

, , +13

A Method for Gene Disruption That Allows Repeated Use of URA3 Selection in the Construction of Multiply Disrupted Yeast Strains

Eric Alani, Lei Cao, Nancy Kleckner · Genetics · 1987 · 883 citations

, , +15