Emergence of Ofloxacin Resistance in <i>Mycobacterium tuberculosis</i> Clinical Isolates from China as Determined by <i>gyrA</i> Mutation Analysis Using Denaturing High-Pressure Liquid Chromatography and DNA Sequencing

Abstract

A high rate of double point mutations in gyrA (56% of 87 ofloxacin-resistant Mycobacterium tuberculosis clinical isolates) indicates the emergence of fluoroquinolone resistance. This is the first report to describe denaturing high-pressure liquid chromatography analysis of mutations in gyrA of M. tuberculosis in a large number of clinical isolates.