THE DETERMINATION OF NUCLEOTIDE PHOSPHORUS WITH A STANNOUS CHLORIDE - HYDRAZINE SULPHATE REAGENT

Abstract

Stabilization of stannous chloride as reducing agent for the analysis of phosphorus by the molybdenum blue method has been achieved by combining it with hydrazine sulphate. Application of the procedure to the determination of inorganic and nucleotide phosphorus is described. Colorimetric measurement of light absorption is made at 700 mμ, permitting a highly sensitive and accurate estimation of phosphorus.