INTRAMOLECULAR DISTRIBUTION OF URIC ACID-N15 AFTER ADMINISTRATION OF GLYCINE-N15 AND AMMONIUM-N15 CHLORIDE TO GOUTY AND NONGOUTY SUBJECTS*

Abstract

Data on the intramolecular distribution of the isotope incorporated into uric acid in normal man were recorded in one subject given glycine-N15 by Shemin and Rittenberg (12) who found that most of the uric acid-N15 appeared at N-7 but that a substantial proportion, increasing from 23 per cent on day 1 to 41 per cent on day 14, was present also in the remaining nitrogens, 1, 3, and 9 (Figure The N15 en- richment of the three remaining nitrogen atoms implied that appreciable quantities of nitrogen de- rived from glycine are utilized in normal man to provide N-9 and N-3 by way of the amide nitro- gen of glutamine, and N-1 through the amino nitrogen of aspartic acid (11) (Figure