Direct Detection of Dermatophytes in Skin Samples Based on Sequences of the Chitin Synthase 1 (CHS1) Gene.

Abstract

For the direct detection of dermatophytes in skin scrapings and hairs from animals, a primer pair specific to the chitin synthase 1 (CHS1) gene of dermatophytes was constructed. By PCR analysis with the primer pair, dermatophyte DNA could be diagnosed directly and rapidly in clinical skin samples.