Factors Affecting Protein–Glycan Specificity: Effect of Spacers and Incubation Time

Abstract

Abstract Glycan binding : We monitored the binding of synthetic glycans to influenza hemagglutinin by using ELISA and surface plasmon resonance, thereby demonstrating that the glycan's presentation influences binding dramatically. Also, the binding observed in static systems was very different from that in dynamic fluid systems. These studies suggest that binding specificities are dependent on glycan structure, valency, presentation, and assay conditions. magnified image