ELECTROPHORETIC PROFILES OF AQUEOUS‐SOLUBLE PROTEINS OF HUMAN CEREBRAL CORTEX: POPULATION AND DEVELOPMENTAL CHARACTERISTICS¹

Abstract

Abstract —Aqueous‐soluble brain proteins from 100 adult humans have been screened by discontinuous polyacrylamide and by continuous‐SDS‐polyacrylamide electrophoresis for evidence of genetically determined variation. In addition 42 adult Macaca nemestrina and 36 inbred strains of mice were screened in the SDS‐continuous system. No variation in position of major bands was found within species, although differences in relative intensity of staining were noted. Fetal human cerebral cortex gave profiles of aqueous‐soluble proteins distinguishable from that of adults. After affinity chromatography on Concanavalin‐A‐agarose, adult samples gave one band on SDS‐polyacrylamide gels, while fetal samples gave the same band plus an additional band, presumably representing glycoproteins.