Method for determination of partition coefficients by high-performance liquid chromatography on an octadecylsilane column. Examination of its applicability.

Abstract

The high-performance liquid chromatography (HPLC) of various compounds on an ODS (octadecylsilane) stationary phase was carried out with mobile phases containing vaious amounts of organic solvents, and the retention behavior was analyzed in terms of the partition coefficient between octanol and water P<oct>. It was found that the capacity factor k' increased linearly with Poct. However, this relation was dependent on the hydrogen-bonding ability of the compounds. The retention behaviors of non-hydrogen bonders, hydrogen acceptors and amphiprotic compounds were different.It was shown that from the results of HPLC on an ODS column, values of Poct of various compounds can be determined accurately and simply, by a single equation, when the hydrogen-bonding property of the compounds is take into account. The accuracy of the estimated Poct value increased with decrease in the concentration of the organic solvent in the mobile phase.