Detection of N-hydroxy-2-acetylaminofluorene—DNA adducts in rat liver measured by radioimmunoassay

Abstract

Antibodies to imidazole ring opened derivatives of alkali treated N-(guanosin-8-yl)-2-aminofluorene (1-[6-(2,5-diamino-4-oxopyrimidinyl-N6-riboside)]-3-(2-fluorenyl)urea were elicited in rabbits by immunization with a conjugate between the ring-opened derivative and bovine serum albumin. The specificity of the antibodies was studied by radioimmunoassay. These antibodies and antibodies to N-(guanosin-8-yl)-N-acetyl-2-aminofluorene were used to titrate the adducts formed in liver DNA of rats treated with N-hydroxy-2-acetylaminofluorene. The ring-opened derivatives represent approximately 10% of the dGuo-C8-adducts.