Improved method for the automatic determination of serum inorganic phosphate.

Abstract

Inorganic phosphate is separated from protein by dialysis and allowed to react with ammonium molybdateatpH4-0. The colourless phosphomolybdic acid which is formed is reduced by metol (p-methylaminophenol sulphate) to a blue complex which is measured colorimetrically. REAGENTS SAMPLE DILUENT NaCl, 9-0 g., per litre distilled water with 10 drops 10% Brij (Honeywell & Stein, Ltd.) added.