Design and Fabrication of PLGA Sandwiched Cell/Fibrin Constructs for Complex Organ Regeneration

Abstract

A poly(DL-lactic-co-glycolic acid) (PLGA) sandwich fibrinogen/ adipose stem cell (ADSC) construct was fabricated to generate smooth muscle tissue. The mechanical properties and ADSC compatibility of PLGA, poly(ethylene glycol-1,6-hexamethyl diisocyanate-caprolactone) i.e. polyurethane (PU), gelatin, alginate, and fibrin composites were evaluated for vascular smooth muscle tissue generation. Synthetic PLGA and PU combined with natural gelatin, alginate, and fibrin for strength and cell compatibility were found to be effective. A trilayer construct was designed and built with a microporous inner PLGA layer to provide nutrient, oxygen, and metabolite transfer while the outer PLGA layer with no pores prevented leakage during in vitro culture and in vivo implantation. The fibrin layer suitably accommodated ADSC growth, migration, proliferation, and differentiation inside the construct. This design has the potential for wide use in tissue engineering and complex organ construction.