CD4 T‐lymphocyte determinations on whole blood specimens using a single‐tube three‐color assay

Abstract

Abstract We evaluated a flow cytometrïc method for determining the proportion of CD4‐positive T lymphocytes in whole blood using a single three‐color tube containing fluorochrome‐labeled CD45, CD3, and CD4. Various ways of gating this sample were evaluated and results were compared with data obtained in our standard six‐tube, two‐color assay gated on light scatter parameters. Excellent correlation was found between the three‐color analysis using a CD45/side scatter gate and the standard two‐color analysis with a light scatter gate. This single‐tube three‐color test is a promising assay for monitoring CD4 + T‐lymphocytes. © 1993 Wiley‐Liss, Inc. This article is a US Government work and, as such, is in the public domain in the United States of America.