Phytopathology · 1992 · 76 citations · 0 references
AgricultureGeneticsPlant SciencesPlant BiotechnologyPlant GeneticsTransgenic CropsAgrobacterium-mediated TransformationPlant TransformationPlant Virus ResistancePlant VirusesResistance Breeding For Viral DiseasesVirus Genome OrganizationAgrobacterium TumefaciensAgrobacterium-mediated Transformation ConstructsExpression Cassette DesignGene Expression Cassette DesignTomato WiltTransgenic Virus ResistanceVirus DiseasesVirus-resistant Plants
The nucleocapsid protein (NP) gene of the lettuce isolate of tomato spotted wilt virus (TSWV-BL), from the L serogroup, was synthesized and cloned into a plant expression cassette using polymerase chain reaction. Transgenic tobacco plant lines were obtained via Agrobacterium tumefaciens-mediated leaf disk transformation, and their progenies were tested for their ability to resist infections by TSWV isolates belonging to the L and I serogroups (...)