ZOOLOGICAL SCIENCE · 2013 · 14 citations · 19 references
EngineeringBioengineeringBiomedical EngineeringRegenerative MedicineStem Cell BiologyTissue EngineeringBone RegenerationBone Tissue EngineeringCell CryopreservationCell-based TherapiesMesenchymal Stem Cell TherapyOsteogenesisBone Marrow Mesenchymal Stem CellsCell Viability AssaysLong-term Storage StrategiesMesenchymal Stromal Cell CryopreservationOsteogenic DifferentiationOsteogenic MarkersStem Cell StorageStem Cell-based Therapy
Autologous bone marrow-derived mesenchymal stem cell (BMSCs)-based therapies show great potential in regenerative medicine. However, long-term storage and preservation of BMSCs for clinical use is still a great clinical challenge. The present study aimed to analyze the effect of long-term cryopreservation on the regenerative ability of BMSCs. After cryopreservation of BMSCs from beagle dogs for three years, cell viability, and quantitative analysis of alkaline phosphatase (ALP) activity, surface adherence, and mineralized nodule formation were analyzed. BMSCs in cell-scaffold complex were then implanted into nude mice. There was no significant difference in cell viability and ALP activity between osteogenic differentiation and non-osteogenic differentiation of BMSCs, and BMSCs in cell-scaffold complex retained osteogenic differentiation ability in vivo. These results indicate that long-term cryopreserved BMSCs maintain their have capacity to contribute to regeneration.
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Su‐Hwan Kim, Kyoung‐Hwa Kim, Byoung‐Moo Seo et al. · Journal of Periodontology · 2009 · 185 citations · Full text
Niklas Rye Jørgensen, Zanne Henriksen, O. H. Sørensen et al. · Steroids · 2004 · 173 citations
Steroid effects on osteogenesis through mesenchymal cell gene expression
Xudong Li, Li Jin, Quanjun Cui et al. · Osteoporosis International · 2004 · 148 citations · Full text