Optogenetic characterization methods overcome key challenges in synthetic and systems biology

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

Lei S. Qi, Matthew H. Larson, Luke A. Gilbert et al. · Cell · 2013 · 5K citations · Full text

Off-target Effect, Engineering, Rna-guided Platform +9

Millisecond-timescale, genetically targeted optical control of neural activity

Edward S. Boyden, Feng Zhang, Ernst Bamberg et al. · Nature Neuroscience · 2005 · 4.8K citations

Neural Mechanism, Optical Manipulation, Neuroscience +4

A synthetic oscillatory network of transcriptional regulators

Michael B. Elowitz, Stanislas Leibler · Nature · 2000 · 4.7K citations

Transcriptional Regulation, Signal Transduction, Engineering +9

Construction of a genetic toggle switch in Escherichia coli

Timothy S. Gardner, Charles R. Cantor, James J. Collins · Nature · 2000 · 4.3K citations

Engineering, Genetic Engineering, Molecular Biology +7

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

Luke A. Gilbert, Matthew H. Larson, Leonardo Morsut et al. · Cell · 2013 · 3.7K citations · Full text

Transcriptional Regulation, Natural Sciences, Molecular Biology +6