Antimicrobial Agents and Chemotherapy · 1994 · 15 citations · 17 references
Life SciencesBiochemistryMolecular BiologyEnzymologyMolecular VirologyVirologyAntiviral PharmacologyEnzyme ExpressionEnzyme KineticsRna TranscriptionViral TranscriptionVirus PurificationEscherichia ColiPharmacological SpecificityRecombinant Enzyme ExpressionRecombinant Enzyme IsolationRecombinant Enzyme ProductionRecombinant Enzyme SystemsViral PolymerasesViral Rna Transcription
Reverse transcriptase from feline immunodeficiency virus (FIV) has been cloned and expressed in Escherichia coli. We have purified this recombinant enzyme and shown that it is a 66-kDa protein that is indistinguishable from virion-derived FIV reverse transcriptase in sensitivity to the 5'-triphosphates of 3'-azido-3'-deoxythymidine and the four 2',3'-dideoxynucleosides. The availability of large quantities of the FIV reverse transcriptase will allow more detailed physical and pharmacological studies.
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
Ulrich K. Laemmli · Nature · 1970 · 251.6K citations