The calmodulin-ubiquitin (CUB) genes of Trypanosoma cruzi are essential for parasite viability

Molecular Cloning. A Laboratory Manual

EJ Wood · Biochemical Education · 1983 · 30.6K citations

Life Sciences, Molecular Biology, Molecular Genetics +13

[21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction

Kary B. Mullis, Fred Faloona · Methods in enzymology on CD-ROM/Methods in enzymology · 1987 · 5.7K citations

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Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.

Peter J. Southern, Paul Berg · PubMed · 1982 · 2.8K citations

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Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae

Linda Gritz, Julian Davies · Gene · 1983 · 813 citations

Life Sciences, Microbiology, Molecular Biology +14

Plasticity in chromosome number and testing of essential genes in Leishmania by targeting.

Ângela K. Cruz, Richard G. Titus, Stephen M. Beverley · Proceedings of the National Academy of Sciences · 1993 · 203 citations

Life Sciences, Genetics, Molecular Genetics +14