Science · 1991 · 981 citations · 27 references
AgriculturePlant BiologyPlant SciencesPlant BiotechnologyPlant GeneticsPlant-microbe InteractionsBreeding For Fungal Disease ResistanceFungal PathogenesisPlant Disease ResistancePlant PathogenesisPlant-pathogen InteractionsPlant-pathogenic FungiDefense Response MechanismsFungal Cell WallsHost Defense EnhancementHost Defense ResponsesPlant Disease Resistance MechanismsPlant Disease SymptomsSoil-borne Fungal PathogensTransgenic Plants
The production of enzymes capable of degrading the cell walls of invading phytopathogenic fungi is an important component of the defense response of plants. The timing of this natural host defense mechanism was modified to produce fungal-resistant plants. Transgenic tobacco seedlings constitutively expressing a bean chitinase gene under control of the cauliflower mosaic virus 35S promoter showed an increased ability to survive in soil infested with the fungal pathogen Rhizoctonia solani and delayed development of disease symptoms.
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A Simple and General Method for Transferring Genes into Plants
R. Horsch, J. E. Fry, Nancy Hoffmann et al. · Science · 1985 · 4.8K citations
Antifungal Hydrolases in Pea Tissue
Félix Mauch, Brigitte Mauch‐Mani, Thomas Boller · PLANT PHYSIOLOGY · 1988 · 1.2K citations · Full text
A general method for site-directed mutagenesis in prokaryotes
Gary Ruvkun, Frederick M. Ausubel · Nature · 1981 · 698 citations