Relationship between Ni(II) and Zn(II) Coordination and Nucleotide Binding by the Helicobacter pylori [NiFe]-Hydrogenase and Urease Maturation Factor HypB

Abstract

Background: Helicobacter pylori HypB (HpHypB) is a metal-regulated GTPase essential for the biosynthesis of [NiFe]hydrogenase and urease. Results: Nickel binding to HpHypB is altered upon nucleotide binding, an effect not observed with zinc. Conclusion: Metal coordination and the GTPase cycle of HpHypB are intimately linked. Significance: These data suggest that HpHypB contributes to metal fidelity in [NiFe]-hydrogenase and urease biosynthesis.