PROCEDURE FOR EMBEDDING IN SITU SELECTED CELLS CULTURED IN VITRO

Abstract

Ultrastructure studies of cells in vitro are greatly facilitated when the procedures used permit combined light and electron microscopy of selected cells or cellular components. The methods currently available for such studies are designed primarily for pre-selection of cells and are not suitable for high resolution light microscope observation. Such techniques are further limited because they require special treatment, such as growing cell monolayers on carbon films (1). The present report describes a simplified flatface embedding method which can be used to study individual chromosomes, nucleoli, or other cellular components with both the light and electron microscope. The method is now used routinely