Journal of the American Chemical Society · 2008 · 319 citations · 7 references
Immunocytochemical TechniqueEngineeringCirculating Tumor CellsHomogeneous DetectionImmunologyBlood CellPathologyImmunophenotypingSurface-enhanced Raman ScatteringWhole BloodBiomedical EngineeringLight Scattering SpectroscopyHuman Whole BloodHematologySerologic TestingMagnetic BeadsClinical ChemistryLaboratory MedicineBiophysicsTumor MicroenvironmentSingle-molecule DetectionBiomedical ImagingDirect EnumerationMedicineSpectroscopic Method
A novel, homogeneous SERS-based cell detection assay was developed for rapid and direct enumeration of circulating tumor cells in the presence of whole blood. Magnetic beads and SERS tags were respectively conjugated to EpCAM and her2 antibodies for the capture and detection of approximately 50 tumor cells/mL in the presence of whole blood in less than 1 h.
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