DNA Profiling. An Approach of Systemic Characterization, Classification, and Comparison of Genomic DNAs

Abstract

Abstract A new method to obtain profiles of giant genome DNAs is described. It is based on two key techniques: Random PCR using oligonucleotides and temperature gradient gel electrophoresis. The experiments applied to the DNAs of 3 strains of E.coli demonstrated that this technique is informative and useful. The random PCR which uses various combinations of oligonucleotides as primers is effective to extract specific DNAs from a giant genome DNA.